Lady chatlines lover - Prospects for elucidating the phylogeny of the apicomplexa
The Apicomplexa is the only large taxonomic group whose members are entirely parasitic and is, therefore, presumably of major interest to parasitologists.
We might, for example, expect that we know a great deal about the biology of the group by now and that we have a clear phylogenetic framework within which to organize that knowledge.
All members of this phylum have an infectious stage—the sporozoite—which possesses three distinct structures in an apical complex.
To provide a foundation for such research areas, in the present study, we (i) sequenced the nuclear genome of ], and genomic DNA was isolated using the Gentra Puregene kit (Qiagen, Hilden, Germany) and total RNA employing Tri Pure (Sigma-Aldrich, St Louis, MI, USA), according to the manufacturers’ protocols.
The nucleic acids were quantitated using a fluorometer (Qubit Invitrogen, Carlsbad, CA, USA), and their quality was verified using a Bio Analyzer (2100, Agilent).
Babesiosis is a socioeconomically important tick-borne disease of animals (including humans) caused by haemoprotozoan parasites.
The severity of babesiosis relates to host and parasite factors, particularly virulence/pathogenicity. This disease has a major, adverse economic impact on the health and productivity of livestock animals, particularly ruminants, as a consequence death, reduced meat and milk production, increased sterility and abortion rates and/or the cost of treatment and prevention [ spp.
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A total of 13 haemogregarine haplotypes were identified, which fell into four main clades in a phylogenetic framework.
Phylogenetic analysis of six new lankesterellid haplotypes revealed that these parasites were distinct from, but phylogenetically related to, known Lankesterella species and might represent new taxa.
For example, ]; this alteration can be accompanied by an accumulation of affected erythrocytes in the capillaries of organs, including the brain and lungs, leading to severe cerebral disease, respiratory insufficiency and/or multi-organ failure.